Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer’s Disease: In Vitro and In Vivo Evidence

Table 1

Experimental conditions for immunofluorescence.

Primary antibody	Brand primary antibody	Primary antibody dilution	Secondary antibody	Brand secondary antibody
Rabbit α-GFAP	Abcam	1 : 1000 0.5% BSA in TBS/0.25% triton X-100	FITC conjugated goat anti-rabbit IgG (H+L) 1 : 200, 0.5% BSA in TBS/0.25% triton X-100	Jackson ImmunoResearch
Rabbit α-S100B	Novus Biologicals	1 : 250 0.5% BSA in TBS/0.25% triton X-100	TRITC conjugated goat anti-rabbit IgG (H+L) 1 : 200, 0.5% BSA in TBS/0.25% triton X-100	Jackson ImmunoResearch
Mouse α-MAP2	Novus Biologicals	1 : 250 0.5% BSA in TBS/0.25% triton X-100	TRITC conjugated goat anti-mouse IgG (H+L) 1 : 200, 0.5% BSA in TBS/0.25% triton X-100	Jackson ImmunoResearch