Palmitoylethanolamide Dampens Reactive Astrogliosis and Improves Neuronal Trophic Support in a Triple Transgenic Model of Alzheimer’s Disease: In Vitro and In Vivo Evidence

Table 2

Primer sequences used for RT-PCR.

Sequence of interest	Primer 5→3		Annealing temperature (°C)	Number of cycles
GFAP	Forward	GAAGAGGGACAACTTTGCAC	61	32
	Reverse	GCTCTAGGGACTCGTTCGTG
S100B	Forward	TAATGTGAGTGGCTGCGGAA	63	32
	Reverse	CCTCACCAAGGGCTAAGCAG
iNOS	Forward	CAAGCTGATGGTCAAGATCCAGAG	64	40
	Reverse	GTGCCCATGTACCAACCATTGAAG
COX-2	Forward	GCTGTACAAGCAGTGGCAAA	62	30
	Reverse	CCCCAAAGATAGCATCTGGA
GAPDH	Forward	GCTACACTGAGGACCAGGTTGTC	64	30
	Reverse	CCATGTAGGCCATGAGGTCCAC