Protective Effects of Lycium barbarum Extracts on UVB-Induced Damage in Human Retinal Pigment Epithelial Cells Accompanied by Attenuating ROS and DNA Damage

Figure 7

Activation of genes involved in L. barbarum extract-mediated protective effect on UVB irradiation. The human global gene expression profiles of RNA were isolated from ARPE-19 cells pretreated with L. barbarum extracts LBE or LBA prior to the irradiation of UVB then analyzed by a cDNA microarray at 24 hr. (a) A total of 403 genes in LBE and a total of 558 genes in LBA were changed, and 308 genes might be involved in the signaling pathways associated with the protective effect of L. barbarum extracts using the analysis of IPA bioinformatics software. (b). Top-level functional annotation categories of genes including peroxisome proliferator-activated receptor (PPAR), the nuclear receptor as a transcription factor, Integrin, and Toll-like receptor (TLR) signaling pathways were affected by the pretreatment of both L. barbarum extracts LBE and LBA. Remarkably, the activation level of the TLR pathway is higher in the LBE pretreatment than in the LBA pretreatment. (c) Genes might be involved in the activation of the TLR pathway. A pink color indicates the upregulation of the genes. A green color indicates the downregulation of the genes.

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