Transient Receptor Potential Canonical Channels 4 and 5 Mediate Escherichia coli-Derived Thioredoxin Effects in Lipopolysaccharide-Injected Mice

Figure 4

Effects of bacterial thioredoxin (Trx) in the number of peritoneal leukocytes and macrophage-mediated phagocytosis in SIRS mice. Bacterial Trx (20 μg/150 μl/mouse, subcutaneously, twice a day, for 3 days prior to lipopolysaccharide (LPS) injection) effects in the numbers of peritoneal (a) mononuclear and (c) polymorphonuclear (PMN) cells and (e) macrophage-mediated phagocytosis in TRPC5^+/+ and TRPC5^−/− mice injected with vehicle (PBS; ) or LPS (). Effects of bacterial Trx (20 μg/150 μl/mouse, subcutaneously, twice a day, for 3 days prior to LPS injection) in the numbers of peritoneal (b) mononuclear and (d) polymorphonuclear (PMN) cells and (f) macrophage-mediated phagocytosis in SIRS and non-SIRS mice treated with vehicle (6% DMSO in PBS; ) or the TRPC4/TRPC5 antagonist ML204 (1 mg/kg; ) subcutaneously, twice a day, for 5 days prior to LPS injection. ML204-treated mice were used for comparison (). differs from vehicle-injected controls.

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