Transient Receptor Potential Canonical Channels 4 and 5 Mediate Escherichia coli-Derived Thioredoxin Effects in Lipopolysaccharide-Injected Mice

Table 1

Inflammatory mediator levels in peritoneal lavage samples obtained from TRPC5^+/+ and TRPC5^−/− mice intraperitoneally (i.p.) injected with lipopolysaccharide (LPS) and pretreated subcutaneously with either vehicle (PBS; ) or bacterial thioredoxin (Trx; 20 μg/150 μl, twice a day, for 3 days, ). Animals treated with vehicle (PBS) were used as controls (). differs from the vehicle + vehicle group; differs from the vehicle + LPS group; differs from LPS-injected WT mice pretreated with bacterial Trx.

Inflammatory mediator	TRPC5^+/+			TRPC5^−/−
	Vehicle + vehicle	Vehicle + LPS	Trx + LPS	Vehicle + vehicle	Vehicle + LPS	Trx + LPS
TNFα (pg/ml)	47.0 ± 2.7	68.6 ± 5.7	87.3 ± 14.4	49.3 ± 10.3	78.0 ± 8.6	119.0 ± 12.7
IL-6 (pg/ml)	1339.0 ± 69.0	9822.0 ± 1969.0	7957.0 ± 2141.0	1311.0 ± 38.7	13319.0 ± 4011.0	29657.0 ± 4882.0
IL-10 (pg/ml)	254.5 ± 3.3	309.7 ± 12.1	354.2 ± 66.4	274.8 ± 29.2	329.1 ± 19.4	367.0 ± 36.1
NO^x (μM)	5.5 ± 1.2	5.3 ± 1.2	9.3 ± 1.1	6.3 ± 0.9	7.5 ± 0.7	27.7 ± 10.7
H2O2 (μM)	2.5 ± 0.6	4.3 ± 0.8	7.5 ± 0.9	3.1 ± 0.7	3.8 ± 0.8	10.8 ± 4.4