Transient Receptor Potential Canonical Channels 4 and 5 Mediate Escherichia coli-Derived Thioredoxin Effects in Lipopolysaccharide-Injected Mice

Table 2

Inflammatory mediator levels in peritoneal lavage samples obtained from C57BL/6 mice intraperitoneally (i.p.) injected with lipopolysaccharide (LPS) or vehicle (PBS) and pretreated subcutaneously with vehicle (6% DMSO in PBS; ), ML204 (1 mg/kg, 150 μl/mice, twice a day, for 6 days; ), and/or bacterial thioredoxin (Trx, 20 μg/150 μl, twice a day, for 3 days; ). differs from the vehicle + vehicle group; differs from the vehicle-control group treated with ML204, Trx or ML204 + Trx; differs from the vehicle + LPS group.

Inflammatory mediator	Vehicle + vehicle	ML204 + vehicle	Trx + vehicle	ML204 + Trx + vehicle	Vehicle + LPS	ML204 + LPS	Trx + LPS	ML204 + Trx + LPS
TNFα (pg/ml)	8.8 ± 2.6	15.5 ± 2.5	16.1 ± 2.6	33.2 ± 3.5	16.3 ± 5.7	14.3 ± 8.1	7.8 ± 3.3	7.3 ± 3.1
IL-6 (pg/ml)	2.1 ± 0.4	2.0 ± 0.7	1.8 ± 0.4	2.9 ± 0.7	403.0 ± 133.1	144.0 ± 61.1	105.2 ± 55.0^&	186.9 ± 94.3
IL-10 (pg/ml)	4.8 ± 2.5	12.0 ± 1.4	9.9 ± 1.6	20.7 ± 1.3	8.4 ± 2.2	1.7 ± 0.8	1.6 ± 0.6	6.3 ± 2.7
NO^x (μM)	47.3 ± 7.3	0.1 ± 0.03	9.0 ± 7.1	6.1 ± 6.0	96.9 ± 10.2	122.9 ± 24.0	78.2 ± 8.0	81.7 ± 8.4
H2O2 (μM)	16.5 ± 4.7	30.9 ± 3.4	19.7 ± 4.6	8.2 ± 3.0	19.4 ± 4.1	19.1 ± 5.2	10.6 ± 2.4	12.3 ± 2.8