Polypharmacological Profiles Underlying the Antitumor Property of Salvia miltiorrhiza Root (Danshen) Interfering with NOX-Dependent Neutrophil Extracellular Traps

Figure 4

Sal B and DHT I selectively inhibit specific NET-associated enzymes. (a) The effects of Sal B and DHT I on citH3 by Western blot analysis. Protein levels were quantified with NIH ImageJ software and were normalized with GAPDH. Graphs show mean ± SD from two independent blots. between control and PMA, while between PMA and PMA plus drug treatment. The effects of Sal B and DHT I on recombinant PAD4 activity (b) or NE (c) or MPO (d) using fluorescence-based methods provided by commercial kits. The potential effects were expressed either 100% inhibition or 100% activity normalized to vehicle controls in triplicate.

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