Polypharmacological Profiles Underlying the Antitumor Property of Salvia miltiorrhiza Root (Danshen) Interfering with NOX-Dependent Neutrophil Extracellular Traps

Figure 6

Danshen and its components promote the spontaneous apoptosis of neutrophils in vitro. (a) The effects of Sal B, DHT I, Sal B plus DHT I, or DSI on the NETotic activity of neutrophils for 12- and 24-hour treatments by plate reader assay. or as shown in the indicated comparison. (b) Nuclear morphology after 24-hour drug treatment was assessed by cell morphology at 400x magnification, scale bar represents 50 μM. (c) The effects of Sal B, DHT I, Sal B plus DHT I, or DSI on neutrophil apoptosis for 12- and 24-hour treatments by flow cytometry after Annexin V/PI labeling. Samples were run in duplicate, and 10,000 events were captured within the neutrophil gate each time. The percentage of apoptotic cells (%) was cells labeled with Annexin V (+)/PI (−) and Annexin V (+)/PI (+). as shown in the indicated comparison.

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