Research Article
Phagocytosis of Candida albicans Inhibits Autophagic Flux in Macrophages
Figure 1
Candida albicans treatment reduces autophagic flux. (a) THP-1-derived macrophages were treated with C. albicans in the presence or absence of chloroquine (CQ) for the indicated time. The LC3-II/β-actin ratio decreased after treatment with C. albicans. A significant difference in the LC3-II/β-actin ratio was not observed between dimethyl sulfoxide- (DMSO-) treated and untreated cells. (b, c, d) THP-1-derived macrophages were treated with C. albicans in the presence or absence of E-64d+ pepstatin, NH4Cl, and BAF-A1 for the indicated period. The LC3-II/β-actin ratio decreased after treatment with C. albicans. (e, f) RAW 264.7 macrophage-like cells were treated with C. albicans in the presence or absence of NH4Cl/BAF-A1 for 8 hours. The LC3-II/β-actin ratio was decreased after treatment with C. albicans. Bars represent the mean ± SEM of independent experiments performed in triplicate (; ).
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