Phagocytosis of Candida albicans Inhibits Autophagic Flux in Macrophages

Figure 6

LAP induced by Candida albicans influences ROS production, apoptosis, and proliferation of THP-1-derived macrophages. (a) After treatment for 8 hours, proliferation of THP-1-derived macrophages was detected using the BrdU assay. The asterisks indicate significant differences. C1M, the C. albicans strain name. (b) THP-1-derived macrophages were treated with rapamycin in the presence or absence of C. albicans for 8 hours. Western blotting was used to determine the levels of caspase-3. (c, d) After treatment for 8 hours, cells were collected to detect apoptosis and death. The ratio of apoptotic cells was calculated, and there was no difference between groups. (e, f) After treatment for 8 hours, ROS production was assayed by flow cytometry. The mean fluorescence intensity (MFI) of DCFH-DA was analyzed, and the asterisks indicate significant differences. Bars represent the mean ± SEM of independent experiments (, , and ).

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