Di-2-pyridylhydrazone Dithiocarbamate Butyric Acid Ester Exerted Its Proliferative Inhibition against Gastric Cell via ROS-Mediated Apoptosis and Autophagy

Figure 7

DpdtbA-induced change in lysosomal membrane permeability and cathepsin D translocation. LysoTracker Red-stained MGC-803 cells (objective size 10 × 10): (a) control, (b) 2.5 μM DpdtbA, and (c) 5.0 μM DpdtbA. The enhanced fluorescence intensities of the cells clearly indicated the alteration of LMP. Immunofluorescence detection of cathepsin D in MGC-803 cells (objective size 10 × 20): (d) control cells and (e) 2.5 μM DpdtbA. (f) Western blotting analysis of cathepsin D in cytosol.

(a)

(b)

(c)

(d)

(e)

(f)