Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII

Figure 2

Characterization of C-kit⁺ CSCs, exosomes, and cellular internalization. (a) Purified cells were double stained for c-kit (green) and DAPI (blue) and observed under a fluorescence microscope (Olympus, Japan). (b) Representative FCM characterization of C-kit⁺ CSCs for typical surface antigens and isotype control after magnetic bead sorting. Surface expression of C-kit, and the absence of surface expression of CD45 and CD34. (c) Transmission electron microscopy analysis of BMSC-exos. Scale bar = 100 nm. (d) NTA of exosome diameter and concentration. (e) Western blotting of the exosome markers CD63, CD9, and Alix. (f) CSCs were incubated with DiI-labeled BMSC-exos (400 μg/ml) for 24 h. Fluorescence photomicrographs showed internalized DiI-labeled BMSC-exos (red) in DAPI-labeled CSCs (blue). Scale bar = 20 μm.

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