Polysiphonia japonica Extract Attenuates Palmitate-Induced Toxicity and Enhances Insulin Secretion in Pancreatic Beta-Cells

Figure 3

Polysiphonia japonica extract (PJE) protects against palmitate-induced β-cell dysfunction in zebrafish. (a) Zebrafish were incubated with 10 μg/mL PJE and 0.2 mM palmitate (PA) from 3 to 4 days postfertilization (dpf). PJE was added 1 h prior to PA treatment. Thereafter, the zebrafish incubated with 3 or 20 mM glucose for 3.5 h. (b) Confocal microscopy images of the pancreas of zebrafish. Scale bar: 100 μm. (c) Relative EGFP fluorescence levels from (b). (d) Number of β-cells per embryo from (b). –6. and . n.s.: no significance.

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