Enhancing TFEB-Mediated Cellular Degradation Pathways by the mTORC1 Inhibitor Quercetin

Figure 4

Effects of quercetin on mTOR activity in RPE cells. Cells were pretreated with 10 μM quercetin for 16 hr and stimulated with either amino acids (a), insulin, or serum (b) for 30 min. Phosphorylation of S6 and Akt was measured by Western blot analyses as indicators of mTOR kinase activity. Amino acids (almost all) used were a mixture of essential amino acids used in Minimum Essential Medium (MEM), including glutamine. Basal: cells incubated in serum-free medium.

(a)

(b)