Evodiamine Attenuates P2X₇-Mediated Inflammatory Injury of Human Umbilical Vein Endothelial Cells Exposed to High Free Fatty Acids

Figure 6

Effects of EVO on the levels of ERK 1/2, p-ERK 1/2, and TNF-α in HUVECs. HUVECs were treated with control (1% BSA) or high FFAs (1 mM) in the absence or presence of 0.25 μM EVO for 3 d. (a) Gel images of Western blotting show the expression of ERK 1/2 and p-ERK 1/2. (b) Bar graph indicates the IOD ratio of ERK 1/2 to β-actin. (c) Bar graph indicates the IOD ratio of p-ERK 1/2 to ERK 1/2. (d) Western blotting images show the expression of TNF-α. (e) Bar graph indicates the IOD ratio of TNF-α to β-actin. The data are mean ± SEM of three independent experiments in triplicate. versus control group and versus high FFA group.

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