Rutin Isolated from Chrozophora tinctoria Enhances Bone Cell Proliferation and Ossification Markers

Table 2

Proliferative effects of compounds isolated from C. tincturia: doubling time assessment.

	MCF-7	SAOS2	MG-63
Control	16.6 ± 1.4	49.3 ± 4.1	36.8 ± 2.2
1	9.3 ± 0.2	26.3 ± 1.4	20.9 ± 0.3
2	14.8 + 1.7	35.7 ± 3.4	27.5 ± 2.1
3	8.6 ± 0.4	14.7 ± 0.7	21.0 ± 0.3
4	11.8 ± 3.6	29.0 ± 2.9	25.8 ± 3.5
5	13.4 ± 3.2	29.4 ± 3.5	23.5 ± 1.1
6	7.2 ± 0.5	15.7 ± 0.2	20.8 ± 0.5
E2	8.5 ± 0.4	20.1 ± 1.2	23.1 ± 0.1

Cells were treated with test compounds (1 μM) for up to 96 h and viability was determined using SRB assay. Doubling times were calculated and compared to control untreated cells and E₂ (0.1 μM) treated cells (positive control). Data are expressed as mean ± SD; . Significantly different from the corresponding untreated cells; .