Attenuation of High-Fat Diet-Induced Rat Liver Oxidative Stress and Steatosis by Combined Hydroxytyrosol- (HT-) Eicosapentaenoic Acid Supplementation Mainly Relies on HT

Figure 2

Liver oxidative stress-related parameters in mice subjected to control diet (CD) and high-fat diet (HFD) without (−) and with eicosapentaenoic acid (EPA), hydroxytyrosol (HT), and EPA + HT supplementation. Antioxidant capacity of plasma (a) and contents of total GSH equivalents (b), GSH (c), GSSG (d), GSH/GSSG ratios (e), TBARs (f), F-8 isoprostanes (g), and protein carbonyls (h). Values are for 8 animals per experimental group. Groups sharing the same symbol are not significantly different among them according to two-way ANOVA and the Bonferroni posttest (). GSH: reduced glutathione; GSSG: glutathione disulphide; TBARs: thiobarbituric acid reactants. ^, and indicate the significant differences between the experimental groups.

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