Evaluation of Potential Mechanisms Controlling the Catalase Expression in Breast Cancer Cells

Figure 2

DNA damage pathway did not enhance catalase expression in breast cancer cell lines. (a and b) Immunoblotting and protein quantification of p53, P-p53, and P-Chk2 in breast cancer cells. Ascorbate 1 mM/menadione 10 μM (A/M) for 2 h is used as a positive control to induce DNA damage. (c) mRNA levels of the different kinases activated during the DNA damage pathway. (d) c-abl mRNA level in both MCF-7 and Resox cells. (e and f) Immunoblotting and protein quantification of c-abl in breast cancer cells. Data are mean ± s.e.m. Groups were compared using unpaired t-test. value <0.05; value <0.01; value <0.001.

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