Evaluation of Potential Mechanisms Controlling the Catalase Expression in Breast Cancer Cells

Figure 3

Catalase is neither phosphorylated nor ubiquitinated in breast cancer cells. (a) Catalase protein levels were measured after 5 h incubation with proteasome inhibitor MG132 in both MCF-7 and Resox cells. (b) Immunoprecipitation (IP) with anticatalase antibody and immunoblotting (IB) with anti-Flag, antiphosphocatalase, and catalase antibodies. Prior IP, cells were transfected with a plasmid pcDNA3 (1 μg) coding an ubiquitin-Flag for 72 h.

(a)

(b)