Evaluation of Potential Mechanisms Controlling the Catalase Expression in Breast Cancer Cells

Figure 4

Evaluation of mRNA stability in MCF-7 and Resox cells. DNA methyltransferase inhibitor 5-aza-2-deoxycytidine tends to increase the levels of catalase protein level in MCF-7 cells. (a) Catalase mRNA levels were measured in MCF-7 and Resox cells after 2, 4, and 6 h of actinomycin D treatment (10 μg/ml). (b and c) Immunoblotting and protein quantification of catalase, after 72 h incubation with 1 μM of 5-aza-2-deoxycytidine (Aza-DC). (d and e) Cells were prior incubated with 1 μM of 5-aza-2-deoxycytidine for 72 h, then with various concentrations of ascorbate (Asc, mM) and menadione (Men, μM). Cell survival was measured by MTT assay. Data are mean ± s.e.m. Groups were compared using unpaired t-test. value <0.05; value <0.01.

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