Research Article

Protective Effects of a Polyphenol-Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress-Induced Diabetic Rats

Figure 3

LC-MS/MS analysis of PESc polyphenol content. RP-HPLC-MS/MS chromatographic profile of PESc (a) was obtained. PESc was analyzed by MS/MS using the MRM method. Gallic acid was detected by using m/z 169/125, m/z 169/97, and m/z 169/79. For myricetin, MRM transitions corresponded to m/z 317/271, m/z 317/151, and m/z 317/179 while for glycosylated forms of myricetin m/z 449/316, m/z 449/271, and m/z 449/179. Finally, quercetin was followed by the m/z 301/151 and m/z 301/179. Chemical identification of the structure of the compounds present in PESc was analyzed by mass spectrometry. Representative structures and fragmentation patters of gallic acid (b); myricetin-3-α-arabinopyranoside (c), myricetin deoxyhexoside (d), myricetin (e), and quercetin (f) are shown. Data is representative of at least three independent experiments.