Protective Effects of a Polyphenol-Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress-Induced Diabetic Rats
Inhibition of LOX by PESc. Lipoxygenase activity was followed by formation of conjugated dienes at 234 nm (ε = 2.5 × 10−4 M−1·cm−1). Activity was determined from the initial linear slope of changes in absorbance. Enzyme protein concentration was determined and specific activity in the absence or presence of the extract or the purified fractions reported. Results are expressed as the remaining initial activity in the presence of the different inhibitors’ concentrations. (a) Dose-dependent action of myricetin (MYR) on LOX activity. (b) LOX activity was determined at different PESc concentrations in the absence (closed squares) or presence (open circles) of MYR, to detect if an additive or cooperative action was observed. (c) The obtained IC50 values in each condition are reported. In all cases, data are presented as mean ± SD of three independent experiments at least in triplicate for each condition.