Exendin-4 and Liraglutide Attenuate Glucose Toxicity-Induced Cardiac Injury through mTOR/ULK1-Dependent Autophagy

Figure 2

Effect of exendin-4 (Exe) and liraglutide (LIRA) on accumulation of ROS and mitochondrial O₂⁻ as well as antioxidant Nrf2: (a) representative images of DCF staining depicting the effect of Exe and LIRA on high glucose-induced ROS production in H9c2 cells, scale ; (b) pooled data of DCF quantification, ; (c) quantification of MitoSOX red intensity, ; (d) levels of Nrf2 normalized to GAPDH. Inset: representative gel blots of Nrf2 and GAPDH using specific antibodies, , independent cell cultures per group, , versus the NG group and versus the HG group.

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