Exendin-4 and Liraglutide Attenuate Glucose Toxicity-Induced Cardiac Injury through mTOR/ULK1-Dependent Autophagy

Figure 6

Effect of exendin-4 (Exe) and liraglutide (LIRA) on glucose toxicity-induced loss of GFP-LC3 puncta; (a) representative images of GFP-LC3, DAPI staining and merged images of GFP-LC3, and DAPI staining in H9c2 cells cultured in normal-glucose or high-glucose medium treated with or without Exe, LIRA, the autophagy inhibitor 3MA, or the autophagy inducer rapamycin; (b) quantitative analysis of cells with GFP-LC3 positive puncta. , fields of independent cultures per group, versus the NG group, versus the HG group and versus Exe or LIRA-treated HG group.

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