Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 1

Research Article

Modulation of Oxidative Status by Normoxia and Hypoxia on Cultures of Human Dermal Fibroblasts: How Does It Affect Cell Aging?

Figure 1

Cellular proliferation analysis in HDF under 5% O2 and 21% O2 culture conditions. (a) Growth curve of young HDF cells cultured under 5% O2 (grey bar) and 21% O2 (black bar). The points correspond to 24–48–72 h of culture and indicate fold increase in cell number. Mean values were calculated on at least six replicates. (b) The doubling time of young HDF grown under the two different oxygen tensions (). (c) Proliferation index of the cells cultured under 5% and 21% O2 calculated between day 0 and day 3 after CFSE staining in both young (Y) and old (O) cells. Error bars represent ± SD. ; ; .

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