Oxidative Medicine and Cellular Longevity / 2018 / Article / Fig 4

Research Article

Modulation of Oxidative Status by Normoxia and Hypoxia on Cultures of Human Dermal Fibroblasts: How Does It Affect Cell Aging?

Figure 4

Superoxide anion level and ROS production in HDF during cellular aging under 5% O2 and 21% O2 culture conditions. (a) Flow cytometric analysis of mitochondrial superoxide anion production in young (Y) and old (O) HDF under 5% O2 and 21% O2 culture conditions determined using the MitoStress Kit. In the right panel, the superoxide anion level is reported as % of positive cells. (b) Flow cytometric analysis of intracellular levels of ROS in young (Y) and old (O) HDF under 5% O2 and 21% O2 determined using the carboxy-H2DCFDA assay. In the right panel, ROS production is reported as % of positive cells, obtained by subtraction of PI from carboxy-H2DCFDA fluorescence. In the left panel, representative cytometric histograms are reported for young cells (white) and old cells (grey) under the two oxygen tensions (21% and 5% O2). Error bars represent ± SEM. ; .

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