Intravitreal Injection of Hydrogen Peroxide Induces Acute Retinal Degeneration, Apoptosis, and Oxidative Stress in Mice

<div>Apoptosis analysis in the retina after the H<sub>2</sub>O<sub>2</sub> treatment. (a) The schematic diagram of apoptosis analysis after the H<sub>2</sub>O<sub>2</sub> treatment. (b) No TUNEL-positive cells were found in the saline group. After H<sub>2</sub>O<sub>2</sub> treatment, TUNEL-positive cells were found in both the ONL and INL in each group and sparely found in GCL in the 10 <i>μ</i>g H<sub>2</sub>O<sub>2</sub> group. (c) The number of TUNEL-positive cells increased after the H<sub>2</sub>O<sub>2</sub> treatment in the ONL, INL, and GCL. (d) The proportions of TUNEL-positive cells showed an increase after H<sub>2</sub>O<sub>2</sub> treatment in the ONL, INL, and GCL. <span class="nowrap"><svg height="13.531973pt" id="M48" style="vertical-align:-3.429431pt" version="1.1" viewbox="-0.049816 -10.102542 50.605198 13.531973" width="50.605198pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 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173C361 105 336 22 258 22C176 22 126 120 126 240C126 266 127 291 132 310C155 323 189 337 227 337Z"></path></g></svg> from 3 mice). Scale bar: 50 <i>μ</i>m.</div>

Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: Intravitreal Injection of Hydrogen Peroxide Induces Acute Retinal Degeneration, Apoptosis, and Oxidative Stress in Mice 