Research Article

The Secretion from Neural Stem Cells Pretreated with Lycopene Protects against tert-Butyl Hydroperoxide-Induced Neuron Oxidative Damage

Figure 5

Ly-NSC-CM protects cultured neurons against t-BHP-induced apoptosis. (a) Immunofluorescence imaging of neurons labeled with cleaved caspase-3 (red) and DAPI (blue). (b) Quantification of cleaved caspase-3 labeling. The histogram displays the proportion of cleaved caspase-3-positive cells (as a % of total cells) in each group. (c) Representative images of TUNEL-positive cells (green) and DAPI (blue). (d) Quantification of the TUNEL assay. The histogram displays the percentage of TUNEL-positive cells (as % of total cells) in each group. . Data are described as mean ± SEM. versus control group. versus t-BHP group. versus NSC-CM group.
(a)
(b)
(c)
(d)