Research Article
The Impact of Moderate Chronic Hypoxia and Hyperoxia on the Level of Apoptotic and Autophagic Proteins in Myocardial Tissue
Figure 4
DNA fragmentation and apoptosis: (a) expression level of the ratio of Bax to Bcl2 after exposure to 10% O2, 21% O, and 30% O2 for 28 days (, 6, and 6, resp.); (b) representative picture of Bax and Bcl2; (c) results of TUNEL assay, percent of the ratio of Tdt+ to total nuclei (, 4, and 4, resp.). The inset reports high, positive linear correlation; (a) the linear regression yielded , correlation coefficient , value = 0.0072 and when significant is displayed with the 95% CI (dotted curves) and the slope . (c) The inset report the one-way ANOVA and Tukey multiple comparison post hoc test. ; ; . (d) Representative immunofluorescence pictures obtained in heart tissue samples from hypoxia (10% O2), normoxia (21% O2), and hyperoxia (30% O2) for 28 days.
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