Mito-TEMPO Alleviates Renal Fibrosis by Reducing Inflammation, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress

Figure 6

The effect of Mito-TEMPO on 5/6 nephrectomy-induced oxidative stress. The SOD2 activity (a) and MDA level (b) in isolated kidney mitochondria, serum T-SOD (c), and MDA (d) levels were measured following the manufacturer’s protocol. Mitochondrial superoxide was detected by MitoSOX Red fluorogenic dye and images were taken with Nikon fluorescent microscope (e) (magnification, ×200), and the mean MitoSOX fluorescence intensity per image was calculated by Image Pro Plus software and normalized to the control group (f). Western blot analysis of Sirt3, SOD2, and AcSOD2 (g). Relative expression of AcSOD2 (h) normalized to the expression of SOD2 and Sirt3 (i) normalized to the expression of β-actin. Data are presented as mean ± SD (). , control group versus CKD group; , CKD group versus CKD + MT1 and CKD + MT3 groups.

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