The Role of Nrf2 Signaling in PPARβ/δ-Mediated Vascular Protection against Hyperglycemia-Induced Oxidative Stress

Figure 2

Effect of PPARβ/δ agonist, GW0742, on Nrf2 target gene induction. (a, b) mRNA and (c) protein expression of NOQ-1 and HO-1. HUVECs, exposed to low- (LG) or high-glucose (HG) medium for 24 h, were coincubated with GW0742 (GW) alone or preincubated with the GSK0660 (GSK) followed by GW0742. mRNA data presented as a ratio of arbitrary units of mRNA (2^−ΔΔCt). All data are mean ± SEM (), and experiments were repeated at least three times independently. Protein data presented as densitometric values and protein band normalized to the corresponding α-actin; the bands are representative of –5. and versus LG. versus HG. versus GW column.

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