The Role of Nrf2 Signaling in PPARβ/δ-Mediated Vascular Protection against Hyperglycemia-Induced Oxidative Stress

Figure 3

Effect of PPARβ/δ agonist, L165041, on Nrf2 target genes. (a, b) mRNA and (c) protein expression of NOQ-1 and HO-1. HUVECs, exposed to low- (LG) or high-glucose (HG) medium for 24 h, were coincubated with L165041 (L) alone or preincubated with the GSK0660 (GSK) followed by L165041. mRNA data presented as a ratio of arbitrary units of mRNA (2^−ΔΔCt). All data are mean ± SEM (), and experiments were repeated at least three times independently. Protein data presented as densitometric values and protein band normalized to the corresponding α-actin; the bands are representative of –5. and versus LG. and versus HG. versus L column.

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