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Oxidative Medicine and Cellular Longevity
Volume 2018, Article ID 5895439, 9 pages
Research Article

Age-Dependent Protective Effect of Selenium against UVA Irradiation in Primary Human Keratinocytes and the Associated DNA Repair Signature

1University Grenoble Alpes, 38000 Grenoble, France
2Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Nanosciences et Cryogénie (INAC), Systèmes Moléculaires et NanoMatériaux pour l'Energie et la Santé (SyMMES), Lésions des Acides Nucléiques (LAN), 17 avenue des martyrs, 38054 Grenoble Cedex, France
3Labcatal Pharmaceuticals, Montrouge, France
4Department of Dermatology and Venereology, CHU Grenoble, Grenoble, France

Correspondence should be addressed to W. Rachidi; rf.aec@idihcar.dilaw

Received 14 March 2017; Revised 19 October 2017; Accepted 3 December 2017; Published 22 February 2018

Academic Editor: Gabriele Saretzki

Copyright © 2018 C. Favrot et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Few studies have focused on the protective role of selenium (Se) against skin aging and photoaging even though selenoproteins are essential for keratinocyte function and skin development. To the best of our knowledge, the impact of Se supplementation on skin cells from elderly and young donors has not been reported. Therefore, the main objective of our study was to evaluate the effects of Se supplementation on skin keratinocytes at baseline and after exposure to ultraviolet A (UVA) irradiation. Low doses of Se (30 nM) were very potently protective against UVA-induced cytotoxicity in young keratinocytes, whereas the protection efficiency of Se in old keratinocytes required higher concentrations (240 nM). Additionally, the DNA repair ability of the old keratinocytes drastically decreased compared with that of the young keratinocytes at baseline and after the UVA exposure. The Se supplementation significantly enhanced the DNA repair of 8-oxoguanine (8oxoG) only in the keratinocytes isolated from young donors. Therefore, aged keratinocytes have an increased vulnerability to oxidative DNA damage, and the Se needs in the elderly should be considered. Strengthening DNA repair activities with Se supplementation may represent a new strategy to combat aging and skin photoaging.