BAG3 Protein Is Involved in Endothelial Cell Response to Phenethyl Isothiocyanate

Figure 3

JNK activity is involved in actin remodeling processes during PEITC treatment. (a) The upper panel displays phase-contrast images and confocal analysis of HUVECs treated with (A, E) 0.01% DMSO (control), (B, F) 10 μM of SP600125 (JNK inhibitor) for 1 h (C, G), and 10 μM of PEITC for 2 h and (D, H) pretreated with 10 μM of SP600125 1 h before PEITC administration. Scale bars: and . (b) The levels of GTP-bound Rac1, phospho-JNK, and phospho-c-Jun in lysates of HUVECs, pretreated or not with 10 μM of SP600125 for 1 h, were analyzed in the presence/absence of 10 μM PEITC stimulation for 2 h. The left bar graphs depict densitometric analysis of Rac1 activity, phospho-JNK, and phospho-c-JUN levels. Results were obtained from at least two independent experiments and are expressed as the mean ± SEM. , , , statistically significant differences, compared to DMSO-treated cells (C), were calculated by Student’s t-test for unpaired data.

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