Combined Therapy with Extracorporeal Shock Wave and Adipose-Derived Mesenchymal Stem Cells Remarkably Improved Acute Ischemia-Reperfusion Injury of Quadriceps Muscle

Figure 7

Cellular expression of inflammatory biomarkers in the quadriceps by day 7 after the IR procedure. (a–e) Showing the immunofluorescent (IF) microscopic finding (400x) for identification of Cox-2+ cells in IR-injured quadriceps (green color). Red color indicated Dil-dye-stained ADMSCs. (f) Analytical result of number of Cox-2+ cells. versus other groups with different symbols (†, ‡, §, and ¶), . (g–k) Showing the IF microscopic finding (400x) for identification of CD68+ cells in IR-injured quadriceps (green color). Red color indicated Dil-dye-stained ADMSCs. (l) Analytical result of number of CD68+ cells. versus other groups with different symbols (†, ‡, §, and ¶), . Scale bars in the lower right corner represent 20 μm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test ( for each group). Symbols (, †, ‡, §, and ¶) indicate significance at the 0.05 level. SC = sham control; IR = ischemia-reperfusion; ECSW = extracorporeal shock wave; ADMSC = adipose-derived mesenchymal stem cell.

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