Oxidative Medicine and Cellular Longevity

Research Article

Blood and Tissue Enzymatic Activities of GDH and LDH, Index of Glutathione, and Oxidative Stress among Breast Cancer Patients Attending Referral Hospitals of Addis Ababa, Ethiopia: Hospital-Based Comparative Cross-Sectional Study

Table 5

A one-way ANOVA (post hoc) analysis of serum and tissue parameters in control subjects and pathologically confirmed breast cancer patients participated from five hospitals of Addis Ababa, Ethiopia (), 2015–2017.


Serum and tissue parameters of BCA	Sample	Stages of breast cancer patients
Serum and tissue parameters of BCA	Sample	Stage 0 ()	Stage I ()	Stage II ()	Stage III ()	Stage IV ()

GDH	S	4.6 ± 0.4	4.1 ± 0.5	4.13 ± 0.91	4.2 ± 0.9	3.7 ± 0.45
GDH	T	0.7 ± 0.2^a	1.2 ± 0.6	1.24 ± 0.71	1.9 ± 1.1^a	1.78 ± 1.15
LDH	S	77.7 ± 27.1	80.9 ± 38.7	138.7 ± 61.9	57.6 ± 5.4	90.0 ± 11.7
LDH	T	42.4 ± 4.1^b	63.8 ± 5.1^b	67.7 ± 3.1^b	131.3 ± 8.4^b	341.8 ± 41.4^b
GSH	S	5.4 ± 2.4	5.9 ± 0.5	5.8 ± 1.8	7.0 ± 3.3	6.3 ± 0.6
GSH	T	1.0 ± 0.3	1.2 ± 0.5	2.0 ± 1.9	1.4 ± 1.5	1.5 ± 0.7
GSSG	S	1.1 ± 0.5	1.2 ± 0.1	1.1 ± 0.4	1.4 ± 0.6	1.2 ± 0.1
GSSG	T	0.3 ± 0.1	0.3 ± 0.04	0.2 ± 0.1	0.3 ± 0.1	0.2 ± 0.03
TOS	S	1.4 ± 0.4^c	3.4 ± 1.3^c	2.4 ± 0.7	3.2 ± 1.16^c	2.8 ± 0.6^c
TOS	T	3.2 ± 0.3	2.3 ± 0.4	3.3 ± 1.6	2.5 ± 1.2	2.4 ± 0.8
TAC	S	0.90 ± 0.29	0.84 ± 0.14	0.87 ± 0.21	0.90 ± 0.34	0.77 ± 0.47
TAC	T	1.84 ± 0.23	1.44 ± 0.33	1.86 ± 0.40	1.46 ± 0.59	1.12 ± 0.83
OSI	S	2.47 ± 0.87	2.15 ± 1.29	2.96 ± 1.8	3.57 ± 2.06	3.98 ± 1.23
OSI	T	2.1 ± 0.87	1.13 ± 0.84^d	1.78 ± 0.4	1.69 ± 0.83	3.03 ± 2.9^d
Total protein	S	212.1 ± 14.4	209.7 ± 8.6	197.3 ± 8.6	210.9 ± 11.6	215.3 ± 4.34
Total protein	T	175.9 ± 51.9	193.8 ± 30.7	195.1 ± 6.5	200.8 ± 12.8	212.2 ± 27.3

^aMean difference of GDH between stages 0 and III of tissue sample (), ^bmean difference of LDH between stages IV and 0, I, II, and III of tissue sample (, 0.001, 0.001, and ≤0.05, resp.), ^cmean difference between stages 0 and I, III, and IV () of blood sample, and ^dmean difference of OSI among stages I and IV in tissue of BCA () were statistically significant. NB: measuring units of GDH and LDH are in mU/l, GSH and GSSG were in μM/μg of total protein, TOS is in μmol H₂O₂ Eq/l, TAC is in mmol Trolox Eq/l, and total protein is in μg/ml.