Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin-Induced Apoptosis Mediated through the Akt-mTOR Pathway in Soleus Muscle

Figure 3

sFRP2 treatment decreases caspase-3 activity and inhibits apoptosis. (a) shows representative imaging of soleus muscle. The muscle has been stained with antimyosin (A, E and I), TUNEL to confirm apoptosis (B, F, and J), and DAPI to determine total nuclei (C, G, and K), and the merged images of all staining can be seen (with enlargements, denoted by a red arrow) in (D, H, and L). (b) shows a graph of the quantitative data from immunohistochemistry for the percentage of apoptotic nuclei. (c) shows a stain of the soleus muscle using antimyosin, caspase-3, TUNEL, and DAPI, from left to right. (d) shows the quantitative results from an ELISA kit for caspase-3 activity, a key mediator in apoptosis. Units represented in percentage of apoptotic nuclei in (b) and in arbitrary units for caspase-3 activity in (d). compared to control, and compared to the Dox group. Scale for (a) is 100 μm. Scale for (c) is 50 μm. -6 for apoptotic nucleus percentage, and -8 for caspase-3 activity.

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