Nox2 Activity Is Required in Obesity-Mediated Alteration of Bone Remodeling

Figure 1

The high-fat diet does not alter superoxide production by the bone marrow cells. Superoxide production was measured using bone marrow (BM) cells isolated from wild-type and Nox2-knockout mice fed with either a standard (SD) or a high-fat diet (HFD) for 3 months. BM cells were stimulated with 50 ng/ml PMA, and chemiluminescence was monitored for the indicated time (a). After normalization by subtraction of the zero time value of the chemiluminescence output, the area under the curve was calculated as a measure of total superoxide production (b). Data are expressed as mean ± SEM (, 6 mice/group). Two-way ANOVA followed by post hoc Tukey’s multiple comparison test was used to assess differences among groups with significance defined as . Significantly different from SD-WT. ^#Significantly different from HDF-WT.

(a)

(b)