Nox2 Activity Is Required in Obesity-Mediated Alteration of Bone Remodeling

Figure 3

Effect of Nox2 on high-fat-diet- (HFD-) induced changes in bone marrow adiposity. Eight-week-old WT and Nox2-KO mice were fed with either an SD or an HFD for 3 months. (a) Left femurs were collected after sacrifice and fixed in 10% neutral-buffered saline (NBF). After processing, sections of bone were embedded in paraffin and stained with hematoxylin and eosin (H&E). Representative microphotographs of stained femur sections from each group are shown. Magnification ×100. The arrow shows adipocytes in the bone marrow cavity. Analysis of white areas (adipocytes) inside the bone marrow has been performed using NIH ImageJ software. (b) Right femurs were crushed under liquid nitrogen, and RNA was isolated and analyzed for mRNA levels for Runx2 and PPAR-γ by real-time RT-PCR. mRNA Ct values for these genes were normalized to the housekeeping gene 18S. Data are expressed as mean ± SEM (, 4 mice/group). Two-way ANOVA followed by post hoc Tukey’s multiple comparison test was used to assess differences among groups with significance defined as . ^§Significantly different from SD-WT. ^#Significantly different from HFD-WT. Significantly different from SD-KO.

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