Potential Phototoxicity of Indocyanine Green in Retinal Pigment Epithelial Cells after Angiography under Ambient Illumination

Figure 6

Mitigating effect of blocking green to red light against ICG phototoxicity. (a) Absorption spectrum of PBS-based medium with 10 μg/mL ICG. Solid, dotted, or bold bar under the horizontal axis indicates wavelength ranges in which DM-blue, DM-green, and DM-red block wavelengths correspond to each DM color, respectively. (b) Relative intensity spectrum of light from the fluorescent lamp. (c) Cell death rates in cultures with 10 μg/mL ICG in the dark and under illumination with or without DM after a 24-hour incubation. The cell death rate in each culture was normalized to that of culture under illumination without DM. DM: dichroic mirror. Dunnett’s test: , unpaired Student’s -test: , Student-Newman-Keuls test: .

(a) Absorption spectrum of ICG

(b) Relative intensity spectrum of fluorescent lamp

(c) Cell death