Uric Acid Protects against Focal Cerebral Ischemia/Reperfusion-Induced Oxidative Stress via Activating Nrf2 and Regulating Neurotrophic Factor Expression

<div>Effect of UA on neuronal injury in cerebral cortices in rats. (a) Representative photomicrographs of HE staining. Scale bar in all panels = 50 <i>μ</i>m. (b) Quantification of surviving neuronal cells. (c) Representative photomicrographs of TUNEL staining. Scale bar in all panels = 50 <i>μ</i>m. (d) Quantification of apoptotic cells. (e) Schematic diagram of coronal brain section. Black rectangle in the ischemic ipsilateral parietal cortex of penumbra represents the region selected for histology. Data are means ± SE (<span class="nowrap"><svg height="8.555213pt" id="M22" style="vertical-align:-0.2063942pt" version="1.1" viewbox="-0.049816 -8.348819 27.83682 8.555213" width="27.83682pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.182,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g transform="matrix(.013,0,0,-0.013,21.474,0)"><path d="M285 378C315 398 338 416 353 432C373 451 384 474 384 503C384 579 325 635 236 635H235C182 635 136 610 108 579L65 516L85 496C110 533 150 575 205 575C258 575 300 543 300 481C300 407 232 369 141 339L147 310C163 315 188 321 211 321C268 321 338 284 338 192C338 94 288 40 217 40C160 40 119 68 93 91C85 98 77 97 69 91C60 84 47 71 46 58C44 46 48 35 62 22C75 10 116 -12 162 -12C234 -12 424 62 424 224C424 297 373 359 285 376V378Z"></path></g></svg>).</span> <svg height="9.351179pt" id="M23" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.049816 -8.714179 64.908327 9.351179" width="64.908327pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M435 580C435 624 455 643 538 643V662H232V643C315 643 333 628 333 580V560C190 560 38 496 38 330C38 193 135 103 316 103H333V75C333 38 299 19 232 19V0H538V19C454 23 435 33 435 76V103H460C517 103 581 121 636 156C692 192 731 255 731 330C731 496 574 560 435 560V580ZM433 523C493 523 539 502 572 461S622 372 622 318S603 221 565 188C527 156 483 140 433 140V523ZM335 140C277 140 231 157 197 192C164 227 147 269 147 318C147 370 165 417 201 459C237 502 282 523 335 523V140Z"></path></g><g transform="matrix(.013,0,0,-0.013,10.035,0)"><path d="M435 580C435 624 455 643 538 643V662H232V643C315 643 333 628 333 580V560C190 560 38 496 38 330C38 193 135 103 316 103H333V75C333 38 299 19 232 19V0H538V19C454 23 435 33 435 76V103H460C517 103 581 121 636 156C692 192 731 255 731 330C731 496 574 560 435 560V580ZM433 523C493 523 539 502 572 461S622 372 622 318S603 221 565 188C527 156 483 140 433 140V523ZM335 140C277 140 231 157 197 192C164 227 147 269 147 318C147 370 165 417 201 459C237 502 282 523 335 523V140Z"></path></g><g transform="matrix(.013,0,0,-0.013,20.069,0)"><path d="M600 480C600 590 528 650 384 650H143L137 622C222 614 225 607 210 531L130 127C113 41 106 36 23 28L17 0H294L300 28C204 36 195 42 212 127L243 284L314 263C327 263 339 263 352 264C465 271 600 337 600 480ZM508 481C508 351 402 304 329 304C289 304 265 311 250 317L295 559C302 594 310 606 323 611C335 616 350 619 367 619C455 619 508 573 508 481Z"></path></g><g transform="matrix(.013,0,0,-0.013,31.752,0)"><path d="M512 -3V55L134 254V256L512 456V514L75 281V230L512 -3Z"></path></g><g transform="matrix(.013,0,0,-0.013,43.043,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.307,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,52.282,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,58.545,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> vs. the sham group; 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Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: Uric Acid Protects against Focal Cerebral Ischemia/Reperfusion-Induced Oxidative Stress via Activating Nrf2 and Regulating Neurotrophic Factor Expression 

Figure 2 | Uric Acid Protects against Focal Cerebral Ischemia/Reperfusion-Induced Oxidative Stress via Activating Nrf2 and Regulating Neurotrophic Factor Expression 