mTOR Modulates Methamphetamine-Induced Toxicity through Cell Clearing Systems

Figure 10

mTOR modulates the number and placement of P20S particles. (a) The graph shows the number of total P20S particles per cell in control, following METH 10 μM, rapamycin 100 nM, and asparagine 50 mM, at 72 h. (b) The graph shows the number of P20S-positive vacuoles per cell in control, following METH 10 μM, rapamycin 100 nM, and asparagine 50 mM, at 72 h. (c) The graph shows the number of P20S-positive vacuoles in control and following METH 10 μM, rapamycin 100 nM, and asparagine 50 mM, at 72 h. (d) The graph shows the ratio between the number of P20S particles within vacuoles and cytosolic P20S particles. Values are given as the mean number of P20S particles and vacuoles counted in 50 cells per group. Error bars represent the standard error of the mean. vs. control; vs. control and METH; vs. METH. ASN = asparagine; RAP = rapamycin.

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