mTOR Modulates Methamphetamine-Induced Toxicity through Cell Clearing Systems

Figure 2

METH induces cell death time- and dose-dependently with a maximal effect at the 1 μM and 10 μM doses. (a) Representative H&E-stained picture from controls. (b) Representative H&E-stained picture following 10 μM METH at 72 h. (c) Graph reporting the percentage of giant cells counted in H&E-stained total cells from controls and METH at 72 h. (d) Dose-dependent graph of H&E-stained cells from control and METH at 72 h. (e) Time-dependent graph of H&E-stained cells from control and 10 μM METH-treated cells. (f) Dose-dependent graph of trypan blue-stained cells from control and METH at 72 h. (g) Time-dependent graph of trypan blue-stained cells from control and 10 μM METH-treated cells. (h) Representative micrograph from a control cell. (h) Representative micrograph from a control cell. (i) Representative micrograph from a METH cell at 72 h. (j) Dose-dependent graph of cell death from control and METH at 72 h. (m) Time-dependent graph of cell death from control and 10 μM METH-treated cells. For the graphs in (c)–(g), values are given as the percentage of cell counted in two triplicates (). For the graphs in (j) and (k), values are given as the percentage of cell counted on 5 grids. Error bars represent the standard error of the mean. vs. control, vs. other groups. Arrows point to vacuoles; asterisk () indicates a large vacuole. N = nucleus. Scale bar = 23.4 μm (a, b) and 2 μm (h, i).

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