mTOR Modulates Methamphetamine-Induced Toxicity through Cell Clearing Systems

Figure 8

mTOR inhibition prevents cell death and rescues the amount of APPs induced by METH and the mTOR activator asparagine. (a) Representative micrographs of control and following METH 10 μM, rapamycin 100 nM, and asparagine 50 mM, at 72 h. (b) Graph of the percentage of cell death in control and following METH 10 μM, rapamycin 100 nM, and asparagine 50 mM, at 72 h. (c) Graph of the number of LC3 + P20S-positive vacuoles in control and following METH 10 μM, rapamycin 100 nM, and asparagine 50 mM, at 72 h. For the graph in (b), values are given as the percentage of cell counted on 5 grids. For the graph in (c), values are given as the mean number of LC3 + P20S-positive vacuoles counted in 100 cells per group. Error bars represent the standard error of the mean. vs. control; vs. control and METH; vs. METH. N = nucleus; ASN = asparagine; RAP = rapamycin. Scale bar = 0.5 μm.

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