mTOR Modulates Methamphetamine-Induced Toxicity through Cell Clearing Systems

Figure 9

mTOR modulates the number and placement of LC3 particles. (a) The graph shows the number of total LC3 particles per cell in control, following METH 10 μM, rapamycin 100 nM, and asparagine 50 mM, at 72 h. (b) The graph shows the number of LC3-positive vacuoles per cell in control, following METH 10 μM, rapamycin 100 nM, and asparagine 50 mM, at 72 h. (c) The graph shows the number of LC3-positive vacuoles in control and following METH 10 μM, rapamycin 100 nM, and asparagine 50 mM, at 72 h. (d) The graph shows the ratio between the number of LC3 particles within vacuoles and cytosolic LC3 particles. Values are given as the mean number of LC3 particles and vacuoles counted in 50 cells per group. Error bars represent the standard error of the mean. vs. control; vs. control and METH; vs. METH.

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