Research Article

Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells

Figure 4

Sequential evaluation of ROS levels in THJ-16T and THJ-11T cells. The cells were treated with 100 μM resveratrol for 0 h, 6 h, 12 h, 24 h, and 48 h and stained by DCFH-DA. (a) Flow cytometer determination of intracellular ROS levels in THJ-16T and THJ-11T cells. (b) Demonstration of ROS levels in THJ-16T and THJ-11T cells by fluorescence microscopy. (c) Time-course response of resveratrol-induced mitochondrial superoxide production in THJ-16T cells. All data represent the means ± SD of three independent experiments. and compared with those at 0 h.
(a)
(b)
(c)