The Role of TLR4 on PGC-1α-Mediated Oxidative Stress in Tubular Cell in Diabetic Kidney Disease

Figure 2

Inhibition of TLR4 protects tubular cell injury by regulating mitochondria-related proteins in diabetic dbdb mice. Dbdb mice received an intraperitoneal injection with the vehicle alone (dbdb group) or TLR4 inhibitor TAK242 (dbdb + TAK group). dbm mice were served as a control. (a) A1, A–C: HE staining was done to renal biopsy tissues of the mice (magnification ×200); A1, D–F: IHC for caspase-3 in tubular cells; A1, G–I: IHC for cytochrome C (magnification ×200); A2 and A3: semiquantification of IHC staining of caspase-3 and cytochrome C. (b) B1: Serum blood glucose level; B2: urinary excretion β-NAG levels in different groups. (c) Western blot for the protein expression of TLR4. (d) D1–D4: Western blot for the protein expressions of PGC-1α, caspase-3, and cytochrome C. Data were expressed as ; .

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