The Role of TLR4 on PGC-1α-Mediated Oxidative Stress in Tubular Cell in Diabetic Kidney Disease

Figure 5

Inhibition of TLR4/NF-κB signaling reversed the expression of PGC-1α and caspase-3 and accumulation of ROS in HK-2 cells in HG ambience. HK-2 cells were treated with TAK242 for 2 h prior to HG (30 mM) treatment for 24 h and with parthenolide and HG (30 mM) for 24 h, and samples were collected for RT-PCR (a) and Western blot analysis (b). (c) C1: MitoSOX Red staining for ROS production (magnification ×200). A: 5.5 Glu group, B: 30 Glu group, C: TAK242 + 30 Glu group, and D: parthenolide + 30 Glu group. C2: Quantification of MitoSOX Red staining. The values were expressed as fold change compared to control. Data were expressed as ; and .

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