36H: A Novel Potent Inhibitor for Antimelanogenesis

Figure 2

Inhibitory enzyme kinetic effects of 36H on mushroom tyrosinase. (a) 36H at different concentrations (1, 5, and 10 mM) affects mushroom tyrosinase activity. The concentration of positive control, Kojic acid, is 10 mM. Data are representative of 3 experiments. (b) The inhibition of mushroom tyrosinase enzyme activity of 10 mM. (c) Inhibition effects of 36H at different concentrations (1, 5, and 10 mM) on the activity of mushroom tyrosinase for the oxidation of L-tyrosine. (d) Inhibitory effect of 36H at different concentrations (10, 50, and 100 mM) on mushroom tyrosinase. The data for Lineweaver-Burk plots were obtained as mean values of three independent assays with various concentrations of L-tyrosine (0.125, 0.25, 0.5, 1, and 2 mM) as the substrate.

(a)

(b)

(c)

(d)