Metabolic Alterations in a Slow-Paced Model of Pancreatic Cancer-Induced Wasting

Figure 2

KPC cell injection promotes skeletal muscle atrophy. (a) Gastrocnemius weight normalized on tibial length. (b) H&E representative pictures of muscle sections from control and KPC-injected mice. (c–e) Gastrocnemius myofiber membranes were stained for laminin, pictures of the whole muscle section were acquired, and the cross-sectional area (CSA) was measured (c). (d) Frequency histogram showing distribution of myofiber CSA in control and KPC-bearing mice. . (e) Representative pictures of laminin staining for CSA analysis in control and KPC-bearing mice. (f–i) KPC injection does not upregulate atrogene expression. . (j) Increased protein ubiquitination and AMPK (T172) phosphorylation in mice bearing tumor; blot representative of three independent experiments. All data are shown as means ± SEM. Statistical analyses were conducted using two-tailed t-test. n.s. = nonsignificant.

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